膜辅蛋白抗体操作步骤

　　【产品名称】：膜辅蛋白抗体，Anti-CD46/MCP抗体

　　【产品类型】：一抗

　　【相关标记】: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647

　　【性 状】： Lyophilized or Liquid

　　【浓 度】： 1mg/1ml

　　【亚 型】： IgG

　　【规 格】：0.1ml/100ug，0.2ml/200ug

　　【抗体来源】： Rabbit OR MOUSE

　　【克隆类型】： polyclonal or monoclonal

　　【产品用途】：科研实验，用于免疫组化实验，WB实验、IF、IP、ELISA实验，相应的标记抗体有HRP标记抗体，FITC标记，BIO等。

　　【贮 存】： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

　　Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

　　【详细介绍】：CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in the regulation of the complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, plaets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderay on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, plaets, epithelial and endothelial cells.

　　免疫组化结果分析：

　　1、首先要确定免疫组化技术是否合格，合格的染色结果应该是背景颜色浅淡或无着色，而阳性标志物清晰和定位明确。

　　切片中的正常组织可以作为最简便的对照来判断染色结果的可靠与否，例如用Vimentin抗体，间质成纤维细胞利血管壁平滑肌细胞胞浆应呈阳性。肯定为阳性的对照组织却呈阴性，待测组织的染色结果也就不可靠；这现象常是由于抗体失效或过度稀释、组织中抗原严重丢失等。

　　2、不仅要判断所检组织中有或无阳性标记物，还要注意阳性信号的分布部位和形态特征。

　　一般说来，阳性信号应与被测抗原所在的部位一致，大部分细胞抗原位于胞质内，其中亦有区别，有的阳性颗粒弥散于整个细胞的胞质，有的则呈斑块状局限于胞浆某处。还有些抗原同时分布于胞质和胞膜(如胃肠腺癌细胞的EMA)，或核与胞质(如S-100、HMB-45)。同一种抗原在不同类型的细胞可定位不同，因而分布的特点也有一定的鉴别意义。