刺激分子B7-1蛋白抗体操作步骤

【产品名称】：刺激分子B7-1蛋白抗体CD80

【规    格】：0.1ml/0.2ml

【相关标记】：Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin   Cy3   Cy5   Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC

【浓    度】：1mg/1ml

【抗体来源】：Rabbit or Mouse or Goat（咨询，）

【克隆类型】：polyclonal or monoclonal

【产品类型】：一抗

【性    状】：Lyophilized or Liquid

【亚    型】：IgG

【纯化方法】：affinity purified by Protein A

【储 存 液】：0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

【产品应用】：not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

【保存条件】： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍    background:

CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.

刺激分子B7-1蛋白抗体，抗体分子是生物学和医学领域用途zui为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。 随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。 我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

    免疫印迹(immunoblotting)又称蛋白质印迹(Western blotting)，它是根据抗原抗体的特异性结合检测复杂样品中的某种蛋白的方法。该法是在凝胶电泳和固相免疫测定技术基础上发展起来的一种新的免疫生化技术。由于免疫印迹具有SDS-PAGE的高分辨力和固相免疫测定的高特异性和敏感性，现已成为蛋白分析的一种常规技术。免疫印迹常用于鉴定某种蛋白，并能对蛋白进行定性和半定量分析。WB对照系统：阳性对照：有标准品，或阳性血清、阳性上清。阴性对照：测血时用相应小鼠未免疫血清，测培养上清，用无克隆培养上清。空白对照：不加一抗，用1%BSA代替。无关对照（替代对照）：用无关项目一抗，或无关项目的抗体。