双甲基精氨酸水解酶1抗体应用说明

 中文名称  双甲基精氨酸水解酶1抗体
别    名  DDAH; DDAH I; DDAH-1; DDAH1; DDAH1_HUMAN; DDAHI; Dimethylargininase 1; Dimethylargininase-1; Dimethylarginine dimethylaminohydrolase 1; N(G); N(G)-dimethylarginine dimethylaminohydrolase 1; NG NG dimethylarginine dimethylaminohydrolase.          
浓    度  1mg/1ml
规 格  0.2ml/200μg          
抗体来源  Rabbit  
克隆类型  polyclonal
交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep   
产品类型  一抗    
研究领域  心血管 细胞生物 神经生物学 血管内皮细胞  
蛋白分子量  predicted molecular weight: 31kDa
性    状  Lyophilized or Liquid
免 疫 原  KLH conjugated synthetic peptide derived from human DDAH1
亚    型  IgG
纯化方法  affinity purified by Protein A
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蜡切片需做抗原修复）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

双甲基精氨酸水解酶1抗体产品介绍 DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production (1,2). Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation (3). DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA) (4). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues (1).
Function : Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Subunit : Monomer.
Tissue Specificity : Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Similarity : Belongs to the DDAH family.
Database links : UniProtKB/Swiss-Prot: O94760.3