形态发生紊乱关联激活因子1抗体应用方法

　　中文名称    形态发生紊乱关联激活因子1抗体

　　英文名称    DAAM1

　　别    名    DAAM 1; DAAM-1; Dishevelled associated activator of morphogenesis 1; FLJ41657; KIAA0666; DAAM1_HUMAN.

　　供 应 商    远慕生物

　　研究领域    细胞生物  免疫学

　　抗体来源    Rabbit

　　克隆类型    Polyclonal

　　交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,

　　产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 形态发生紊乱关联激活因子1抗体（石蜡切片需做抗原修复）

　　not yet tested in other applications.

　　optimal dilutions/concentrations should be determined by the end user.

　　分 子 量    123kDa

　　细胞定位    细胞浆

　　性    状    Lyophilized or Liquid

　　浓    度    1mg/1ml

　　免 疫 原    KLH conjugated synthetic peptide derived from human DAAM1

　　亚    型    IgG

　　纯化方法    affinity purified by Protein A

　　储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

　　保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

　　形态发生紊乱关联激活因子1抗体产品介绍    background:

　　DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.

　　Function:

　　Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.

　　Subunit:

　　Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA.

　　Subcellular Location:

　　Cytoplasm. Note=Perinuclear.

　　Tissue Specificity:

　　Expressed in all tissues examined.

　　Database links:

　　Belongs to the formin homology family.

　　Contains 1 DAD (diaphanous autoregulatory) domain.

　　Contains 1 FH1 (formin homology 1) domain.

　　Contains 1 FH2 (formin homology 2) domain.

　　Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.