磷酸化真核延伸因子激酶2抗体说明书

中文名称    磷酸化真核延伸因子激酶2抗体    

英文名称    Phospho-EEF2k (Ser366)

别    名    EEF2K (phospho S366); p-EEF2K (phospho S366); Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; eEF 2 kinase; eEF 2K; EEF2K protein; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; kinase eEF2K; SMEF2K.    

供 应 商    远慕生物

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Rat, Dog, Pig, Cow, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化真核延伸因子激酶2抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    80kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human EEF2k around the phosphorylation site of Ser366    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化真核延伸因子激酶2抗体产品介绍    background:

Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors.

Subunit:
Monomer or homodimer.

Similarity:
"Belongs to the protein kinase superfamily. Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain.

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.